Hydrolyzed collagen is a form of collagen broken down into smaller peptide chains. In this study, the antibacterial and antioxidant properties of hydrolyzed collagen derived from fish skin and chicken feet were investigated. Collagen was extracted using an acidic method, and its quality was assessed through FTIR, biuret, ash content, and Kjeldahl tests. Antibacterial effects on Gram-positive and Gram-negative bacteria were evaluated using the disc diffusion method, while antioxidant activity was assessed via the DPPH assay. The results revealed that the extraction efficiency of collagen was 2.5% for fish skin and 2.1% for chicken feet. FTIR analysis confirmed the presence of amide bands A, I, II, and III, while the biuret test validated the presence of proteins in the collagen. Antioxidant activity increased with higher collagen concentrations: at 100 ppm, 31% of DPPH radicals were inhibited, and at 500 ppm, the inhibition reached 58%. Additionally, hydrolyzed collagen at a concentration of 50 mg/mL exhibited significant antibacterial activity against both Gram-positive and Gram-negative bacteria. These findings suggest that hydrolyzed collagen possesses notable antioxidant and antibacterial properties and may have potential anti-cancer effects.